Journal: Scientific reports

Article Title: Halofuginone prevents inflammation and proliferation of high-altitude pulmonary hypertension by inhibiting the TGF-β1/Smad signaling pathway.

doi: 10.1038/s41598-025-88258-z

Figure Lengend Snippet: Fig. 4. Effects of HF on pulmonary vascular proliferation in HAPH rats. (A) α-SMA expressions of representative pulmonary arterioles were stained by IHC in each group of rats (magnification × 100/400 and scale bar = 100 μm). (B–D) Changes of proliferative indicators PCNA, CDK6, Cyclin D1 expressions in lung tissue of rats were detected by WB in each group. (E) Change of antiproliferative indicator p21 expression in lung tissue of rats was detected by WB in each group. Original blots are presented in Supplementary information. The data are expressed as the mean ± SD, n = 3 per group. $P < 0.05, $$P < 0.01, *P < 0.05, **P < 0.01 vs. the Nor group; #P < 0.05 vs. the Hyp group.

Article Snippet: All immunohistochemical (IHC) reagents, RIPA buffer, PMSF, BCA protein assay kit, primary antibodies against α-SMA (Cat. No. 19245), PCNA (Cat. No. 13110), CDK6 (Cat. No. 3136), Cyclin D1 (Cat. No. 55506), Smad2/3 (Cat. No. 8685), p-Smad2/3 (Cat. No. 8828), β-actin (Cat. No. 4970), and Anti-rabbit (Cat. No. 7074)/mouse (Cat. No. 7076) IgG, HRP-linked antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Expressing